2016 ACVIM Forum Research Abstract Program

FDA guidelines to assess accuracy and precision were followed. The in vitro stability of clopidogrel, CAM-D, and clopidogrel acid in both citrate and EDTA feline plasma was evaluated at room temperature, 4°C, À20°C and À80°C at 0, 6, 12, 24, 48, 72 hours, and 7 days. Linear regression was performed on an 8 point standard curve (clopidogrel and CAM-D at 1-100 ng/mL, clopidogrel acid at 0.2-20 µg/mL). To evaluate clinical application, two healthy cats were given clopidogrel (Plavix) at the clinically used dose of 18.75 mg orally once daily. Blood was taken prior to clopidogrel administration and at 20, 40, 60, 90 minutes, 4 hours, 6 hours, 8 hours, and 12 hours post clopidogrel administration. All blood samples were collected aseptically into 2 mL cryotubes containing 10 uL of 500 mM BMAP and 20 uL of 500 mM EDTA and then centrifuged. The supernatant was then transferred to new cryotubes and stored at À80°C. All samples were analyzed using HPLC-MS/MS in duplicate with a standard curve.