Author: Pandya, Gagan A.; Holmes, Michael H.; Sunkara, Sirisha; Sparks, Andrew; Bai, Yun; Verratti, Kathleen; Saeed, Kelly; Venepally, Pratap; Jarrahi, Behnam; Fleischmann, Robert D.; Peterson, Scott N.
Title: A bioinformatic filter for improved base-call accuracy and polymorphism detection using the Affymetrix GeneChip® whole-genome resequencing platform Document date: 2007_11_15
ID: 16tii0ha_3
Snippet: The increased availability of complete DNA sequence data for a large number of reference genomes has elevated the value of resequencing methods. Whole-genome DNA resequencing data offer several advantages over existing DNA-based typing methods. We have exploited the modern high-density oligonucleotide arrays as an alternative to the classical ABI sequencing approach towards achieving whole-genome sequence information. Mockler et al. (2) recently .....
Document: The increased availability of complete DNA sequence data for a large number of reference genomes has elevated the value of resequencing methods. Whole-genome DNA resequencing data offer several advantages over existing DNA-based typing methods. We have exploited the modern high-density oligonucleotide arrays as an alternative to the classical ABI sequencing approach towards achieving whole-genome sequence information. Mockler et al. (2) recently provided an excellent overview of applications of DNA tiling arrays for whole-genome analysis that includes genome resequencing, genotyping and polymorphism discovery. The whole-genome, arraybased resequencing and SNP identification approach is simple and time efficient, enabling high-resolution analysis of a number of strains in a matter of days.
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