2016 ACVIM Forum Research Abstract Program

Food was withheld 18 -24 hours, and a cephalic vein catheter implanted. A venous blood sample was collected and the serum and plasma separated and frozen at À80°C. A glucose tolerance test (glucose 0.5 g/kg) was performed. Biochemical (screening, fasting and 2-hr blood glucose in the glucose tolerance test, triglyceride), hormonal (leptin, adiponectin, leptin:adiponectin ratio, insulin, glucose:insulin ratio) and enzymatic (fPLI, MCP-1) analyses were performed on plasma and serum samples. After extraction, analyses using GC-MS was performed. Linear modeling was used to identify statistically significant (adjusted P-value<0.05) metabolites associated with obesity and whether these responses were affected by sex and age. Pearson correlations were used to identify relationships between metabolites and other parameters.