Comparison Of Four Anti-Avian IgY Secondary Antibodies Used In Western Blot And Dot-Blot ELISA To Detect Avian Bornavirus Antibodies In Four Different Bird Species

Escandon, Paulina; Heatley, J Jill; Berghman, Luc R; Tizard, Ian; Musser, Jeffrey MB

A representation of the dot-blot ELISA signals, the ImageJ graphic results, and the signal intensity values generated for the dot are shown in Figure 3 . Signal intensities of the dotblot ELISA using different secondary antibodies within each bird species sampled are shown in Table 1 . For Blue and gold macaw plasma samples, the mean signal intensity of 19,461 obtained for the positive Blue and gold macaw plasma tested using anti-macaw IgY secondary antibody was significantly greater (P<0.05) than the signal intensity obtained when using any of the other three secondary antibodies. The signal intensity of the dots obtained for positive Blue and gold macaw plasma was significantly greater (P<0.05) than that obtained for negative Blue and gold macaw plasma when using anti-macaw IgY secondary antibody. The signal intensity values obtained for the positive Blue and gold macaw samples did not differ significantly from the negative Blue and gold macaw samples when anti-bird, anti-chicken, or anti-duck IgY secondary antibodies were used.