Selected article for: "protein purity and PVDF membrane"
Author: Escandon, Paulina; Heatley, J Jill; Berghman, Luc R; Tizard, Ian; Musser, Jeffrey MB
Title: Comparison Of Four Anti-Avian IgY Secondary Antibodies Used In Western Blot And Dot-Blot ELISA To Detect Avian Bornavirus Antibodies In Four Different Bird Species
  • Document date: 2019_11_12
    • ID: 2sr6ds4r_34_1
    Snippet: igens may have been present in the PVDF membranes and the NC membrane strips of the Western blot and dot-blot ELISAs, respectively, and able to react with antibodies in the sample. To reduce non-specific signals and background noise in the assays, procedures that will absorb or neutralize E. coli antibodies in plasma samples or that will increase the purity of the recombinant N-protein antigen need to be investigated. However even without these a.....
    Document: igens may have been present in the PVDF membranes and the NC membrane strips of the Western blot and dot-blot ELISAs, respectively, and able to react with antibodies in the sample. To reduce non-specific signals and background noise in the assays, procedures that will absorb or neutralize E. coli antibodies in plasma samples or that will increase the purity of the recombinant N-protein antigen need to be investigated. However even without these additional procedures, the dotblot ELISA readily discriminated between ABV antibody positive and negative samples (Figure 2 ), especially in species most prone to natural infection. This suggests the possibility of developing a rapid, patient-side assay that will detect ABV antibodies in psittacine birds.

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