Author: Escandon, Paulina; Heatley, J Jill; Berghman, Luc R; Tizard, Ian; Musser, Jeffrey MB
Title: Comparison Of Four Anti-Avian IgY Secondary Antibodies Used In Western Blot And Dot-Blot ELISA To Detect Avian Bornavirus Antibodies In Four Different Bird Species Document date: 2019_11_12
ID: 2sr6ds4r_36
Snippet: Our study shows that species-specific anti-IgY secondary antibodies provided more accurate results in serologic assays than the use of commercially available anti-chicken and anti-bird IgY secondary antibodies. Due to the large variety of avian species susceptible to ABV infection, the specificity of the secondary antibody must be considered when developing or performing serologic assays, such as dot-blot ELISA and Western blot......
Document: Our study shows that species-specific anti-IgY secondary antibodies provided more accurate results in serologic assays than the use of commercially available anti-chicken and anti-bird IgY secondary antibodies. Due to the large variety of avian species susceptible to ABV infection, the specificity of the secondary antibody must be considered when developing or performing serologic assays, such as dot-blot ELISA and Western blot.
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