Title: Coronavirus induction of class I major histocompatibility complex expression in murine astrocytes is virus strain specific Document date: 1994_9_1
ID: 4bf8eiix_9
Snippet: Infectious Center Assays. The number of infected astrocytes was determined on day 3 p.i. to provide a measure of the relative efficiency of infection by several MHV strains. Briefly, astrocytes were trypsinized into single cell suspensions and, after washing, were plated on DBT cell monolayers at 0.1, 1, 10, 100, and 1,000 cells/60 mm petri dish. Cells were allowed to attach for 1 h at 37~ before the addition of agarose at 0.6% in IkPMI 1640 supp.....
Document: Infectious Center Assays. The number of infected astrocytes was determined on day 3 p.i. to provide a measure of the relative efficiency of infection by several MHV strains. Briefly, astrocytes were trypsinized into single cell suspensions and, after washing, were plated on DBT cell monolayers at 0.1, 1, 10, 100, and 1,000 cells/60 mm petri dish. Cells were allowed to attach for 1 h at 37~ before the addition of agarose at 0.6% in IkPMI 1640 supplemented with 2% heat-inactivated FCS, 20 mM Hepes, and penicillin/streptomycin. Plaques were counted after 48 h incubation at 37~
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