Author: Liu, Zhida; Zhou, Hang; Wang, Wenjun; Tan, Wenjie; Fu, Yang-Xin; Zhu, Mingzhao
Title: A novel method for synthetic vaccine construction based on protein assembly Document date: 2014_12_1
ID: 2tazu4y6_23
Snippet: ELISA. Microtiter plates (Corning Life Sciences, Tewksbury, MA, USA) were coated with 2 mg/ml (100 ml/well) full-length SpyCatcher or TBEV ED3 protein in carbonate buffer at 0.1 M (pH 9.5) overnight at 4uC. After washing away the unbound proteins, the plates were incubated with PBS containing 5% FBS at 37uC for 1.5 h. Serially diluted serum samples from immunized mice were added and incubated at 37uC for 1.5 h. HRP-labeled anti-mouse IgG was used.....
Document: ELISA. Microtiter plates (Corning Life Sciences, Tewksbury, MA, USA) were coated with 2 mg/ml (100 ml/well) full-length SpyCatcher or TBEV ED3 protein in carbonate buffer at 0.1 M (pH 9.5) overnight at 4uC. After washing away the unbound proteins, the plates were incubated with PBS containing 5% FBS at 37uC for 1.5 h. Serially diluted serum samples from immunized mice were added and incubated at 37uC for 1.5 h. HRP-labeled anti-mouse IgG was used as the detection antibody (Zhong Shan-Golden Bridge Biological Technology Co., Ltd, Beijing, China). The plates were visualized by adding 100 ml TMB (eBioscience) and were read at 450 nm using a SpectraMax Plus (Molecular Devices, Sunnyvale, CA, USA).
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