Title: 2016 ACVIM Forum Research Abstract Program Document date: 2016_5_31
ID: 2y1y8jpx_511
Snippet: For this study, fleas were not speciated before DNA extraction. However, previous studies in both countries suggest C. felis was most likely. The detection sensitivity of the assay was shown to be 3.14 ng total C. burnetii genomic DNA per PCR reaction utilized in all 4 primer sets. While all positive and negative controls performed as expected, none of the 96 pooled flea extracts were positive for C. burnetii DNA......
Document: For this study, fleas were not speciated before DNA extraction. However, previous studies in both countries suggest C. felis was most likely. The detection sensitivity of the assay was shown to be 3.14 ng total C. burnetii genomic DNA per PCR reaction utilized in all 4 primer sets. While all positive and negative controls performed as expected, none of the 96 pooled flea extracts were positive for C. burnetii DNA.
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