2016 ACVIM Forum Research Abstract Program

Overall, dietary sodium level had no significant effect on blood pressure, cardiac function, plasma BNP, serum biochemical profiles or urinalyses. There were no dietary effects or diet by time interactions on bone mineral content or body composition. In conclusion, consumption of a diet containing 0.41 g sodium/100 kcal ME had no adverse effects on blood pressure or cardiac function in healthy adult dogs over a 6-month period. The determination of lactate and glucose threshold in dogs submitted to incremental exercise test (IET) is scarce. This study aimed (1) to determine the lactate and glucose thresholds (2) to investigate the acid-base responses of untrained dogs submitted to incremental exercise test. Fourteen Beagle dogs, 1-2 years old, healthy and untrained were used for determination of lactate and glucose thresholds and submitted to IET using treadmill, with slop from 5 to 7.5% and increasing speeds of 0.5 m/s every 5 minutes. The test was concluded when dogs presented fatigue signals. Blood samples were collected after 90 seconds of each effort step, though venous catheter placed in the jugular vein. The samples used for determination of acid-base alterations were collected in the moments before of IET, immediately and five minutes after the fatigue. Data were submitted to Shapiro-Wilk test, and the Pearson was used to correlate the running velocities corresponding to the lactate and glucose threshold and analysis between moments of assessment was performed by using repeated measures one-way ANOVA (P < 0.05). It was found high correlation between lactate and glucose threshold velocities, r = 0.84 (P < 0.01), and for pH, PCO 2 , HCO 3 À strong ions difference (SID) were found moment effect (P < 0.001). The basal values of pH increased (P < 0.001) in fatigue (7.341 AE 0.02 to 7.390 AE 0.04, respectively), and the PCO 2 presented reduction (P < 0.001) in the fatigue and 5 minutes after fatigue (31.74 AE 5.9, and 37.01 AE 5.8), in comparison with basal value (41.4 AE 2.2). The HCO 3 À decreased in fatigue and 5 minutes after IET (18.2 AE 2.3 and 19.1 AE 2.5) when compared with basal (21.9 AE 1.1). The values of SID showed decreased in fatigue compared to values before exercise (36,2 AE 1,7 e 36,6 AE 2.1) respectively. Running velocities corresponding to the lactate and glucose threshold showed accordance between them, what has been already shown in horses and humans, besides results showed respiratory alkalosis and metabolic acidosis, which is considered a change due to hyperventilation and strenuous anaerobic activity, decreasing PCO 2 concentrations, which leads to increase pH through the increase of the relation of HCO 3 À and PCO 2 . SID showed a metabolic acidosis possibly from lactate values increase. Results of this study suggest that glucose threshold can be used as a marker of aerobics capacity in dogs as it is used in humans and horses, besides, maximum exercises in dogs cause acid basic alterations.