Title: 2016 ACVIM Forum Research Abstract Program Document date: 2016_5_31
ID: 2y1y8jpx_786
Snippet: Equine EPCs were incubated with QD (2 -20 nM) for 12-hr or 24-hr, and intensity of label was assessed with fluorescent microscopy. Cell proliferation of EPCs labeled with QD for optimum time and concentration was then assessed by comparing the number of cell doublings per day (NCD) and the population doubling time (PDT) in labeled and unlabeled cells. EPC function was assessed by comparing uptake of acetylated low-density lipoprotein (DiO-Ac-LDL).....
Document: Equine EPCs were incubated with QD (2 -20 nM) for 12-hr or 24-hr, and intensity of label was assessed with fluorescent microscopy. Cell proliferation of EPCs labeled with QD for optimum time and concentration was then assessed by comparing the number of cell doublings per day (NCD) and the population doubling time (PDT) in labeled and unlabeled cells. EPC function was assessed by comparing uptake of acetylated low-density lipoprotein (DiO-Ac-LDL) and in vitro tubule formation in labeled and unlabeled cells.
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