Selected article for: "amplification efficiency and detection method"
Author: Horiuchi, Sho; Saito, Yuichi; Matsui, Atsuka; Takahashi, Nobumasa; Ikeya, Tomohiko; Hoshi, Eishin; Shimizu, Yoshihiko; Yasuda, Masanori
Title: A novel loop-mediated isothermal amplification method for efficient and robust detection of EGFR mutations
  • Document date: 2020_1_14
    • ID: 4sltubqk_3
    Snippet: Loop-mediated isothermal amplification (LAMP) is a new PCR based method with high levels of specificity and A novel loop-mediated isothermal amplification method for efficient and robust detection of EGFR mutations amplification efficiency and utilizes six primers (15) . This method is performed under isothermal conditions, thereby enabling rapid amplification. Due to the high specificity and rapid detection quality of LAMP, this method has been .....
    Document: Loop-mediated isothermal amplification (LAMP) is a new PCR based method with high levels of specificity and A novel loop-mediated isothermal amplification method for efficient and robust detection of EGFR mutations amplification efficiency and utilizes six primers (15) . This method is performed under isothermal conditions, thereby enabling rapid amplification. Due to the high specificity and rapid detection quality of LAMP, this method has been widely used in the fields of bacteriology (16) and virology (17, 18) . However, to the best of our knowledge, there are very few studies reporting the value of LAMP in determining EGFR mutations. Therefore, the present study aimed to detect EGFR mutations in surgically resected tumor tissues from patients with pulmonary adenocarcinoma using this method, as detection of EGFR mutations is one a key examination for patients with pulmonary adenocarcinoma (4, 5, 7, 9) . In addition, the sensitivity, specificity, positive predictive value, negative predictive value and accuracy of LAMP was evaluated and compared with the Therascreen EGFR PCR kit ® .

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