Author: William Rodriguez; Aman Srivastav; Mandy Muller
Title: C19ORF66 broadly escapes viral-induced endonuclease cleavage and restricts Kaposi’s Sarcoma Associated Herpesvirus (KSHV) Document date: 2018_12_26
ID: 5a4i8v59_22
Snippet: Using RT-qPCR, we quantified the expression of several KSHV viral genes. Viral gene 203 expression in KSHV unfolds as a cascade with the "early" (E) genes expressed right after lytic 204 reactivation, followed by "delayed early" (DE) genes and finally, after viral replication, the "late" 205 (L) genes. We harvested timepoints from 0 to 72h after iSLK.219 reactivation and measured 206 RNA levels of genes representative of each gene class upon knoc.....
Document: Using RT-qPCR, we quantified the expression of several KSHV viral genes. Viral gene 203 expression in KSHV unfolds as a cascade with the "early" (E) genes expressed right after lytic 204 reactivation, followed by "delayed early" (DE) genes and finally, after viral replication, the "late" 205 (L) genes. We harvested timepoints from 0 to 72h after iSLK.219 reactivation and measured 206 RNA levels of genes representative of each gene class upon knock-down of C19ORF66. We 207 observed a shift in viral gene expression with early and delayed early viral genes -but not with 208 the late gene -which were expressed earlier and at higher levels in the C19ORF66 knocked 209 down cells as measured by RT-qPCR (Fig. 5C-E) . Taken together, these results suggest that 210 All rights reserved. No reuse allowed without permission.
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