Author: Avirutnan, Panisadee; Hauhart, Richard E.; Marovich, Mary A.; Garred, Peter; Atkinson, John P.; Diamond, Michael S.
Title: Complement-Mediated Neutralization of Dengue Virus Requires Mannose-Binding Lectin Document date: 2011_12_13
ID: 1x3n5job_27
Snippet: Cell lines, sera, and reagents. BHK21-15 and Vero T144 cells were grown in Dulbecco's modified Eagle's medium (DMEM) (Sigma) supplemented with 10% fetal bovine serum (FBS) (Omega Scientific), 100 IU/ml penicillin, 100 g/ml streptomycin, 10 mM HEPES (pH 7.3), and 10 mM nonessential amino acids (Cellgro) at 37°C. C6/36 Aedes albopictus mosquito cells were grown in Leibovitz-15 (Sigma) medium supplemented with 10% FBS and 10 mM HEPES at 25°C. Fres.....
Document: Cell lines, sera, and reagents. BHK21-15 and Vero T144 cells were grown in Dulbecco's modified Eagle's medium (DMEM) (Sigma) supplemented with 10% fetal bovine serum (FBS) (Omega Scientific), 100 IU/ml penicillin, 100 g/ml streptomycin, 10 mM HEPES (pH 7.3), and 10 mM nonessential amino acids (Cellgro) at 37°C. C6/36 Aedes albopictus mosquito cells were grown in Leibovitz-15 (Sigma) medium supplemented with 10% FBS and 10 mM HEPES at 25°C. Fresh human blood samples were collected in glass tubes and allowed to clot at room temperature for 30 minutes. After centrifugation (830 ϫ g) for 10 minutes at 4°C, sera were aliquoted and frozen at Ϫ80°C. Serum samples were thawed on the day of analysis. Sera from wild-type C57BL/6 mice and congenic complement-
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