Author: Alba Grifoni; John Sidney; Yun Zhang; Richard H Scheuermann; Bjoern Peters; Alessandro Sette
Title: Candidate targets for immune responses to 2019-Novel Coronavirus (nCoV): sequence homology- and bioinformatic-based predictions Document date: 2020_2_20
ID: 8p1agcm2_12
Snippet: In previous experiments, we showed that pools based on similar peptide numbers can be generated by sequential lyophilization (11) . These peptide pools (or megapools) incorporate predicted or experimentally-validated epitopes and allow measurement of magnitude and characterization of the phenotype of human T cell responses in infectious disease indications such as Bordetella pertussis, Mycobacteria tuberculosis, Dengue and Zika viruses (11) (12) .....
Document: In previous experiments, we showed that pools based on similar peptide numbers can be generated by sequential lyophilization (11) . These peptide pools (or megapools) incorporate predicted or experimentally-validated epitopes and allow measurement of magnitude and characterization of the phenotype of human T cell responses in infectious disease indications such as Bordetella pertussis, Mycobacteria tuberculosis, Dengue and Zika viruses (11) (12) (13) (14) . The 2019-nCoV CD4 megapool covers all 10 predicted proteins, with the number of potential epitopes proportional to the size of each protein (Table S4) .
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