Author: Lee, Na-Ra; Kwon, Hyun-Mi; Park, Kkothanahreum; Oh, Sangtaek; Jeong, Yong-Joo; Kim, Dong-Eun
Title: Cooperative translocation enhances the unwinding of duplex DNA by SARS coronavirus helicase nsP13 Document date: 2010_7_29
ID: 1k99yv4i_4
Snippet: In this study, the DNA duplex was chosen as a model substrate, and the unwinding of DNA substrates with different duplex lengths and 5 0 -overhang by this helicase was kinetically investigated. We demonstrated that the length of the 5 0 -overhang, which was used as the loading strand for helicase oligomers, was an important factor dictating the processivity of the DNA unwinding process. Thus, we propose that 'functional interaction' of nsP13 heli.....
Document: In this study, the DNA duplex was chosen as a model substrate, and the unwinding of DNA substrates with different duplex lengths and 5 0 -overhang by this helicase was kinetically investigated. We demonstrated that the length of the 5 0 -overhang, which was used as the loading strand for helicase oligomers, was an important factor dictating the processivity of the DNA unwinding process. Thus, we propose that 'functional interaction' of nsP13 helicase oligomers loaded onto the 5 0 -overhang accounts for the observed cooperative translocation of the helicase in DNA unwinding and processivity, which is similar to the mechanism previously suggested for hepatitis C virus NS3 helicase (26) .
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