Author: Gebhardt, Jordan T; Woodworth, Jason C; Jones, Cassandra K; Tokach, Mike D; Gauger, Philip C; Main, Rodger G; Zhang, Jianqiang; Chen, Qi; DeRouchey, Joel M; Goodband, Robert D; Stark, Charles R; Bergstrom, Jon R; Bai, Jianfa; Dritz, Steve S
Title: Determining the impact of commercial feed additives as potential porcine epidemic diarrhea virus mitigation strategies as determined by polymerase chain reaction analysis and bioassay() Document date: 2018_8_20
ID: 6rlbiukh_16
Snippet: Seventy-eight crossbred, 10-d-old pigs of mixed sex were sourced from a single commercial, crossbred farrow-to-wean herd with no prior exposure to PEDV. Also, upon arrival, fecal swabs were obtained and confirmed negative for PEDV, PDCoV, and transmissible gastroenteritis virus (TGEV) using a qRT-PCR assay. To further confirm PEDV negative status, serum was collected and confirmed negative for PEDV antibody by an indirect fluorescent antibody ass.....
Document: Seventy-eight crossbred, 10-d-old pigs of mixed sex were sourced from a single commercial, crossbred farrow-to-wean herd with no prior exposure to PEDV. Also, upon arrival, fecal swabs were obtained and confirmed negative for PEDV, PDCoV, and transmissible gastroenteritis virus (TGEV) using a qRT-PCR assay. To further confirm PEDV negative status, serum was collected and confirmed negative for PEDV antibody by an indirect fluorescent antibody assay and TGEV antibody by ELISA conducted at the ISU-VDL. Pigs were allowed 2 d of adjustment to the new pens before the bioassay began.
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