Author: Lyoo, Kwang-Soo; Yeom, Minjoo; Kim, Jungho; Kim, Donghyuk; Ha, Gunwoo; Na, Woonsung; Le, Van Phan; Song, Daesub
Title: Development of rapid immunochromatographic strip test for the detection of porcine epidemic diarrhoea virus Document date: 2017_12_2
ID: 4szmu1dh_16
Snippet: Expression of the PEDV N protein was confirmed by immunoblot analysis (Fig 1a) . The binding affinity calculated by SPR analysis of mAb 3B12-1A6 and mAb 1H12-1C6 were KD: 4.91E-09 M (ka: 1.97E+05 1/Ms; kd: 9.67E-04 1/s) and KD: 5.09E-09 M (ka: 6.00E+04 1/ Ms; kd: 3.05E-04 1/s), respectively. The kinetics of the mAbs to bind PEDV were similar in the examination. A competition ELISA was performed to test whether the mAbs recognise different epitope.....
Document: Expression of the PEDV N protein was confirmed by immunoblot analysis (Fig 1a) . The binding affinity calculated by SPR analysis of mAb 3B12-1A6 and mAb 1H12-1C6 were KD: 4.91E-09 M (ka: 1.97E+05 1/Ms; kd: 9.67E-04 1/s) and KD: 5.09E-09 M (ka: 6.00E+04 1/ Ms; kd: 3.05E-04 1/s), respectively. The kinetics of the mAbs to bind PEDV were similar in the examination. A competition ELISA was performed to test whether the mAbs recognise different epitopes of PEDV. As shown Table 1 , recognition level of mAb 3B12-1A6 to its epitope was not blocked either alone or in combination with different mAb, and binding of mAb 1H12-1C6 to its epitope was also not blocked. These results indicate that both mAbs recognise different epitopes on PEDV.
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