2015 ACVIM Forum Research Abstract Program

Intra-assay and inter-assay CV of urinary active TGF-b1 measurements were 2.3-7.5% (n = 4) and 4.7-20.9% (n = 4) respectively, dilutional parallelism indicated a mean recovery of 91.6% AE 17.5% (n = 4) and spiking with exogenous TGF-b1 resulted in mean recovery of 115.5% AE 12.9% (n = 9). Cross sectional analysis revealed no significant difference in urinary active TGF- In conclusion, no significant difference was found between cats with differing degrees of renal function in the cross sectional study but UTGF:C did increase prior to documented deterioration of renal function in healthy cats which developed CKD in the longitudinal analysis. This suggests urinary TGF-b1 followed longitudinally in an individual cat may indicate the development of CKD in cats, and further investigation into the role of this mediator is warranted. Bleeding is a complication of chronic kidney disease (CKD) in humans, partially caused by platelet dysfunction and abnormal platelet-vessel wall interactions. Dogs with experimentally induced CKD have prolonged buccal mucosal bleeding times, but coagulation and platelet function have not been evaluated. We compared platelet function between healthy dogs and dogs with CKD using the PFA-100 Ã’ and thromboelastography (TEG), and platelet GPIb, GPIIb-IIIa, and p-selectin expression.