Title: 2015 ACVIM Forum Research Abstract Program Document date: 2015_5_27
ID: 3pnuj5ru_811
Snippet: Tissue samples of the right dorsal colon were collected in suspected insulin resistant (n = 4) and normal horses (n = 5) immediately after euthanasia. The colonic mucosa was dissected from the muscular layers and mounted in Ussing chambers. Ussing chambers are used to measure ion transport and transepithelial resistance in gut mucosa, as an indicator of viability and perme-ability. Colonic tissue was exposed to pH 4, 6, or 7 (control) on the muco.....
Document: Tissue samples of the right dorsal colon were collected in suspected insulin resistant (n = 4) and normal horses (n = 5) immediately after euthanasia. The colonic mucosa was dissected from the muscular layers and mounted in Ussing chambers. Ussing chambers are used to measure ion transport and transepithelial resistance in gut mucosa, as an indicator of viability and perme-ability. Colonic tissue was exposed to pH 4, 6, or 7 (control) on the mucosal side and pH 7.0 on the submucosal side. Potential difference (PD) and short circuit current (Isc) were measured every 15 minutes. Tissue transepithelial resistance (TER) was calculated from the open-circuit PD and Isc, using Ohm's Law (R = PD/Isc).
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