Author: Ma, Lili; Du, Hongmei; Chen, Guangdong
Title: Differential network as an indicator of osteoporosis with network entropy Document date: 2018_5_16
ID: 61t686w7_22
Snippet: Reverse transcription-quantitative PCR (RT-qPCR). Venous blood sample of models were collected and total RNA was extract by TRIzol RNA kit (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to the manufacturer's instructions. The concentration and purity of total RNA were measured by ultraviolet spectrophotometer. Reverse transcription into cDNA was then conducted by a reverse cDNA transcription kit (Takara Bio, Inc., Otsu, .....
Document: Reverse transcription-quantitative PCR (RT-qPCR). Venous blood sample of models were collected and total RNA was extract by TRIzol RNA kit (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to the manufacturer's instructions. The concentration and purity of total RNA were measured by ultraviolet spectrophotometer. Reverse transcription into cDNA was then conducted by a reverse cDNA transcription kit (Takara Bio, Inc., Otsu, Japan). SYBR Ex Script RT-PCR kit (Takara Bio, Inc.) was used to detect the expression of target genes and the primers used in this study is shown in Table I .
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