Selected article for: "culture medium and cytopathic effect"
Author: Xia, Shuai; Yan, Lei; Xu, Wei; Agrawal, Anurodh Shankar; Algaissi, Abdullah; Tseng, Chien-Te K.; Wang, Qian; Du, Lanying; Tan, Wenjie; Wilson, Ian A.; Jiang, Shibo; Yang, Bei; Lu, Lu
Title: A pan-coronavirus fusion inhibitor targeting the HR1 domain of human coronavirus spike
  • Document date: 2019_4_10
    • ID: 3c5ab73l_58
    Snippet: The inhibitory activity of peptides against OC43 replication in HCT-8 cells was assessed, as described elsewhere (66) . Briefly, 100 TCID 50 of OC43 was mixed with a test peptide at graded concentration and incubated at 37°C for 30 min. The mixture was then applied in triplicate onto the monolayer of HCT-8 cells grown in a 96-well microtiter plate. On day 5 after infection, viral titer in the culture medium was tested, and TCID 50 was calculated.....
    Document: The inhibitory activity of peptides against OC43 replication in HCT-8 cells was assessed, as described elsewhere (66) . Briefly, 100 TCID 50 of OC43 was mixed with a test peptide at graded concentration and incubated at 37°C for 30 min. The mixture was then applied in triplicate onto the monolayer of HCT-8 cells grown in a 96-well microtiter plate. On day 5 after infection, viral titer in the culture medium was tested, and TCID 50 was calculated on the basis of the cytopathic effect (CPE) (67) . The inhibitory activity of the tested peptide against 229E replication in A549 cells and NL63 replication in LLC-MK2 cells was evaluated in a similar way, as described above.

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