Author: Ivanova, Elena; Berger, Audrey; Scherrer, Anne; Alkalaeva, Elena; Strub, Katharina
Title: Alu RNA regulates the cellular pool of active ribosomes by targeted delivery of SRP9/14 to 40S subunits Document date: 2015_3_11
ID: 64cnoqpi_15
Snippet: 43S and 48S complexes assembled with [ 35 S]-Met-tRNA i Met and [ 32 P]-MVHL-stop mRNA were subjected to centrifugation through 5-25% sucrose gradient containing 20 mM Tris-HCl pH 7.5, 100 mM potassium acetate, 5 mM MgCl 2 , 2 mM DTT in a Beckman SW60 rotor at 60000 rpm for 2 h. Fractions were analyzed by scintillation counting......
Document: 43S and 48S complexes assembled with [ 35 S]-Met-tRNA i Met and [ 32 P]-MVHL-stop mRNA were subjected to centrifugation through 5-25% sucrose gradient containing 20 mM Tris-HCl pH 7.5, 100 mM potassium acetate, 5 mM MgCl 2 , 2 mM DTT in a Beckman SW60 rotor at 60000 rpm for 2 h. Fractions were analyzed by scintillation counting.
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