Author: Ivanova, Elena; Berger, Audrey; Scherrer, Anne; Alkalaeva, Elena; Strub, Katharina
Title: Alu RNA regulates the cellular pool of active ribosomes by targeted delivery of SRP9/14 to 40S subunits Document date: 2015_3_11
ID: 64cnoqpi_44
Snippet: To determine whether Alu RNPs could also block IRESmediated translation initiation, we made reporter constructs containing IRES sequences from the hepatitis C virus (HCV) and the cricket paralysis virus (CrPV) upstream of Fluc. Formation of the 48S complex on HCV IRES is dependent on eIF3 and either eIF2/eIF5 or eIF5B, whereas the CrPV IRES directly recruits the 40S subunit to the start codon without the need for initiation factors (22) ......
Document: To determine whether Alu RNPs could also block IRESmediated translation initiation, we made reporter constructs containing IRES sequences from the hepatitis C virus (HCV) and the cricket paralysis virus (CrPV) upstream of Fluc. Formation of the 48S complex on HCV IRES is dependent on eIF3 and either eIF2/eIF5 or eIF5B, whereas the CrPV IRES directly recruits the 40S subunit to the start codon without the need for initiation factors (22) .
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