Author: William Rodriguez; Aman Srivastav; Mandy Muller
Title: C19ORF66 broadly escapes viral-induced endonuclease cleavage and restricts Kaposi’s Sarcoma Associated Herpesvirus (KSHV) Document date: 2018_12_26
ID: 5a4i8v59_5
Snippet: Here, taking advantage of the ability of the SRE element to block decay from a diverse 64 set of viral endonucleases, we sought to identify novel escaping mRNAs containing SRE or 65 SRE-like elements in the transcriptome. Using comparative RNA-seq, we uncovered a cluster of 66 75 host mRNAs that escape degradation from four herpesviral endonucleases. Similarly to the 67 previously identified SRE-bearing transcripts, these transcripts were spared .....
Document: Here, taking advantage of the ability of the SRE element to block decay from a diverse 64 set of viral endonucleases, we sought to identify novel escaping mRNAs containing SRE or 65 SRE-like elements in the transcriptome. Using comparative RNA-seq, we uncovered a cluster of 66 75 host mRNAs that escape degradation from four herpesviral endonucleases. Similarly to the 67 previously identified SRE-bearing transcripts, these transcripts were spared from a range of viral 68 -but not host -endonucleases, further supporting that our approach successfully identified 69 Simplex 1 (HSV-1) vhs or an empty vector control were generated using Thy1.1-based cell 97 sorting as described before (45). Total RNA was extracted, polyA enriched and cDNA were 98 generated. cDNA libraries were sequenced with a 100-base single-end read on an Illumina 99
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