Selected article for: "cacodylate buffer and electron microscopy"

Author: Song, Jae-Hyoung; Shim, Aeri; Kim, Yeon-Jeong; Ahn, Jae-Hee; Kwon, Bo-Eun; Pham, Thuy Trang; Lee, Jongkook; Chang, Sun-Young; Ko, Hyun-Jeong
Title: Antiviral and Anti-Inflammatory Activities of Pochonin D, a Heat Shock Protein 90 Inhibitor, against Rhinovirus Infection
  • Document date: 2018_5_2
  • ID: 0bwf8f1i_24
    Snippet: To analyze the mouse respiratory tract, we excised the mouse respiratory tract after HRV1B infection with or without pochonin D treatment. The tissues were washed with PBS, and then fixed in 4% glutaraldehyde (Ted Pella, Redding, CA, USA) and 1% paraformaldehyde solution (Electron Microscopy Sciences, Hatfield, PA, USA) in 0.1 M cacodylate buffer (pH 7.4) for 4 h. After fixation, the tissues were rinsed thrice in 0.1 M cacodylate buffer (Sigma-Al.....
    Document: To analyze the mouse respiratory tract, we excised the mouse respiratory tract after HRV1B infection with or without pochonin D treatment. The tissues were washed with PBS, and then fixed in 4% glutaraldehyde (Ted Pella, Redding, CA, USA) and 1% paraformaldehyde solution (Electron Microscopy Sciences, Hatfield, PA, USA) in 0.1 M cacodylate buffer (pH 7.4) for 4 h. After fixation, the tissues were rinsed thrice in 0.1 M cacodylate buffer (Sigma-Aldrich) (pH 7.4), for 10 min. Samples were then sequentially immersed in 60, 70, 80, 90, and 100% ethanol (Merck, Kenilworth, NJ, USA) for 20 min each. Samples were then immersed in ethanol and isoamyl acetate (Sigma-Aldrich) buffer, and dried. The dried samples were observed using a SUPRA55V VP-FESEM (Carl Zeiss) at the Korean Basic Science Institute, Chuncheon.

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