2018 ACVIM Forum Research Abstract Program: Seattle, Washington, June 14 - 15, 2018

Although SDMA is a dialysable biomarker, we highly recommend evaluating it in context with the concurrent URR for a better estimate of actual GFR. Despite its removal by IH, SDMA still is a better biomarker than sCr for evaluating GFR in dogs with CKD. formance. Since few ELISAs have been validated for feline urine, our objective was to evaluate feline-specific ELISA kits for quantification of IL-2, IL-6, and IL-12 in feline urine. Urine was collected from healthy cats evaluated at the Michigan State University Veterinary Medical Center. Normal urine samples were pooled, modified to mimic disease conditions by adding water or hemolyzed blood, and then spiked with high, medium, and low concentrations of recombinant cytokines. Concentrations of IL-2, IL-6, and IL-12 were determined using commercially available feline-specific ELISA kits. The influence of urine matrix variables on test performance was evaluated by spike/recovery tests, linearity testing, and assessment of inter-and intra-assay variation. All assays underestimated high and medium spiked cytokine concentrations in urine as compared to standard assay diluents. Low spiked cytokine concentrations in urine were inconsistently detected. Urine spiked with IL-2, IL-6 and IL-12 achieved maximal quantitative recoveries of 82%, 90%, and 58% respectively. High urine specific gravity contributed significantly to poor IL-2 and IL-12 recovery Feline idiopathic cystitis (FIC) is a common lower urinary tract disease in cats that resembles interstitial cystitis/painful bladder syndrome in people. In both conditions the pathogenesis of the disease is unknown and there is no specific treatment. To investigate the molecular pathways and disease functions potentially involved in the pathogenesis of FIC, we evaluated the bladder mucosa transcriptome of affected and healthy cats. Urinary bladder tissues were collected from 3 healthy cats and from 3 FIC cats representing 3 different histological phenotypes (ulcerative, nonulcerative, and hyperplastic). RNA from bladder tissues was isolated and sequenced using high throughput next generation sequencing. Differential gene expression and molecular pathway and disease factor analysis was performed using Ingenuity Pathway Analysis (IPA) software. A significant level of gene set enrichment (-log [p] > 1.3) was identified in several annotated disease and function categories. The inflammatory response function was up regulated in the non-ulcerative and ulcerative phenotypes (zscores 1.9 and 2.6 respectively), and down regulated in the hyperplastic phenotype (z-score -3.6). The cellular growth and proliferation category was down regulated in the non-ulcerative and hyperplastic phenotypes (z-scores -2.4 and -3.7) and up regulated in the ulcerative phenotype (z-score 6.5). The cell death and survival pathway was down regulated in the non-ulcerative and hyperplastic phenotype (-2.3 and -2.2) and up regulated in the ulcerative phenotype (6.5) . Further investigation of these pathways and other target genes may provide insight into the pathogenesis of FIC and identify new therapeutic targets as well as biomarkers of the disorder for future clinical applications. The aim of this study was to follow-up CKD dogs with IRIS stages 2 or 3 based on serum creatinine concentration, and also to investigate whether and when SDMA is in disagreement with creatinine concentrations for the classification of CKD stage. The study group was composed of twenty-one CKD dogs. CKD do