2018 ACVIM Forum Research Abstract Program: Seattle, Washington, June 14 - 15, 2018

Study was conducted on commercial dairy operation with 1200 milking Holstein cows. Experimental study protocol was reviewed and approved by institutional Animal Care Committee prior to the study start. Forty lactating cows in average 45.8 days postpartum were selected for the study purposes. Inclusion criteria included history of easy calving, no retained placenta, absence of signs of clinical endometritis and average body condition score of 3 (1 to 5 scale). Prior to MCWF treatment, baseline status of uterine lining was determined by cytology examination. Stained slides were evaluated under microscope to determine the percentage of PMNL's per 300 cells. Following cytology assessment, cows were randomly assigned in four experimental groups with 10 cows per group. On day 0, cows in control group were treated IU with 0.33g of oysterglycogen (proven PMNL attractant), dissolved in 60 ml 0.9%NaCl while animals in MCWF groups received IU dose of either 2.5ml, 5ml or 10ml MCWF diluted in 0.9% NaCl to a total volume of 60ml. Twenty-four hours later, uterine cytology sample from same spot (corpus uteri) was taken and subjected to new cytomorphology analysis and PMNL counts.