Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system Document date: 2019_3_18
ID: 6nq7y1qe_13
Snippet: Luciferase luminescence was assayed by the Luciferase Assay System (Promega, Madison, WI, USA) according to the manufacturer's recommendations. In brief, cells were lysed in Cell Culture Lysis Reagent buffer (CLR), and then centrifuged at 12 000 × g for 2 min at 4 • C. Luciferase Assay Reagent (Promega; 100 l/well) diluted at 1:10 for HEK-293 cells and 1:50 for CHO-K1 cells was added to supernatant (20 l/well). Luminescence readout was taken o.....
Document: Luciferase luminescence was assayed by the Luciferase Assay System (Promega, Madison, WI, USA) according to the manufacturer's recommendations. In brief, cells were lysed in Cell Culture Lysis Reagent buffer (CLR), and then centrifuged at 12 000 × g for 2 min at 4 • C. Luciferase Assay Reagent (Promega; 100 l/well) diluted at 1:10 for HEK-293 cells and 1:50 for CHO-K1 cells was added to supernatant (20 l/well). Luminescence readout was taken on a Tristar 2 microplate reader (Berthold, Bad Wildbad, Germany) with a read time of one second per well for HEK-293 cells and 0.1 s for CHO-K1 cells.
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