Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system Document date: 2019_3_18
ID: 6nq7y1qe_23
Snippet: MTase assays. MTase activity assays were performed by combining 0.5 M of enzymes with 1 M of GpppAC 4 or mGpppAC 4 The products were spotted onto polyethylenimine cellulose TLC plates (Macherey Nagel, Düren, Germany), and resolved using 0.65 M LiCl as mobile phase. The radio-labeled caps released by nuclease P1 were visualized using a Fluorescent Image Analyzer FLA3000 phosphorimager......
Document: MTase assays. MTase activity assays were performed by combining 0.5 M of enzymes with 1 M of GpppAC 4 or mGpppAC 4 The products were spotted onto polyethylenimine cellulose TLC plates (Macherey Nagel, Düren, Germany), and resolved using 0.65 M LiCl as mobile phase. The radio-labeled caps released by nuclease P1 were visualized using a Fluorescent Image Analyzer FLA3000 phosphorimager.
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