Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system Document date: 2019_3_18
ID: 6nq7y1qe_71
Snippet: The kinetics of C3P3-G1 gene expression was monitored by quantitative RT-PCR and luciferase assay in HEK-293 cells. Peak of C3P3-G1 enzyme mRNA levels was detected at 24-48 h, whereas C3P3-G1 Firefly Luciferase mRNA and luminescence peaked at 3 days with sustained expression over 4-6 days in HEK-293 cell line ( Figure 9A-C) . The C3P3-G1 expression system produced at peak 6.7-fold more luciferase mRNA, but 2.2-fold lower luciferase luminescence a.....
Document: The kinetics of C3P3-G1 gene expression was monitored by quantitative RT-PCR and luciferase assay in HEK-293 cells. Peak of C3P3-G1 enzyme mRNA levels was detected at 24-48 h, whereas C3P3-G1 Firefly Luciferase mRNA and luminescence peaked at 3 days with sustained expression over 4-6 days in HEK-293 cell line ( Figure 9A-C) . The C3P3-G1 expression system produced at peak 6.7-fold more luciferase mRNA, but 2.2-fold lower luciferase luminescence at peak than standard CMV-driven Firefly Luciferase expression plasmid. This low luminescence:mRNA ratio shows limited translational efficiency of C3P3-G1 Luciferase transcripts, which suggests incomplete maturation of C3P3-driven transcripts in HEK-293 cells.
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