Selected article for: "oligonucleotide probe and specific probe"

Author: Yeung, Siu-Wai; Lee, Thomas Ming-Hung; Cai, Hong; Hsing, I-Ming
Title: A DNA biochip for on-the-spot multiplexed pathogen identification
  • Document date: 2006_9_25
  • ID: 0sg0hv9w_10
    Snippet: An oligonucleotide capture probe specific for E.coli amplicon [pyrrole-5 0 -ACAACACGTTTAGCCTGACC-3 0 (pyrrole-EC), Apibio, France] was first electrochemically polymerized onto two of the four ITO working electrodes. A mixture of 60 mM pyrrole, 20 mM pyrrole-EC, and 0.1 M LiClO 4 was introduced into the microchamber, followed by a cyclic voltammetric scan of the two electrodes between À0.5 and +0.65 V at a scan rate of 50 mV/s for three times. Th.....
    Document: An oligonucleotide capture probe specific for E.coli amplicon [pyrrole-5 0 -ACAACACGTTTAGCCTGACC-3 0 (pyrrole-EC), Apibio, France] was first electrochemically polymerized onto two of the four ITO working electrodes. A mixture of 60 mM pyrrole, 20 mM pyrrole-EC, and 0.1 M LiClO 4 was introduced into the microchamber, followed by a cyclic voltammetric scan of the two electrodes between À0.5 and +0.65 V at a scan rate of 50 mV/s for three times. The microchamber was then washed with deionized water and dried with nitrogen gas. The same procedure was repeated for the other two ITO working electrodes, except an oligonucleotide capture probe specific for B.subtilis amplicon [pyrrole-5 0 -CCTACGGGAGGCAGCAG-3 0 (pyrrole-BS), Apibio, France] was used.

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