Author: Yeung, Siu-Wai; Lee, Thomas Ming-Hung; Cai, Hong; Hsing, I-Ming
Title: A DNA biochip for on-the-spot multiplexed pathogen identification Document date: 2006_9_25
ID: 0sg0hv9w_17
Snippet: After the amplification step, these amplicons hybridize to their corresponding detection electrodes. Next, the hybridized amplicons are labeled with gold nanoparticles via biotinavidin interaction. Finally, silver metal is electrocatalytically deposited onto the gold nanoparticles and the amount is determined by the electrochemical oxidative dissolution technique (10, 12) . The detailed procedure for the sample preparation, target DNA amplificati.....
Document: After the amplification step, these amplicons hybridize to their corresponding detection electrodes. Next, the hybridized amplicons are labeled with gold nanoparticles via biotinavidin interaction. Finally, silver metal is electrocatalytically deposited onto the gold nanoparticles and the amount is determined by the electrochemical oxidative dissolution technique (10, 12) . The detailed procedure for the sample preparation, target DNA amplification, and product detection steps is given in the Materials and Methods section.
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