Selected article for: "detection electrode and single microchamber"
Author: Yeung, Siu-Wai; Lee, Thomas Ming-Hung; Cai, Hong; Hsing, I-Ming
Title: A DNA biochip for on-the-spot multiplexed pathogen identification
  • Document date: 2006_9_25
    • ID: 0sg0hv9w_4
    Snippet: In this work, we present a complete DNA-based assay in a single silicon-glass microchamber for multiple pathogen detection. A model system of E.coli and B.subtilis was used. The assay involves the following steps: (i) sample preparation using thermal cell lysis and magnetic particle-based target genome isolation; (ii) target DNA amplification by the PCR; (iii) hybridization of the amplicons to their complementary oligonucleotide capture probes im.....
    Document: In this work, we present a complete DNA-based assay in a single silicon-glass microchamber for multiple pathogen detection. A model system of E.coli and B.subtilis was used. The assay involves the following steps: (i) sample preparation using thermal cell lysis and magnetic particle-based target genome isolation; (ii) target DNA amplification by the PCR; (iii) hybridization of the amplicons to their complementary oligonucleotide capture probes immobilized onto individual detection electrode surfaces and (iv) electrochemical transduction of the recognition event via gold nanoparticles with signal amplification using electrocatalytic silver deposition (10) . An issue of importance much needed to be addressed was the compatibility of all the materials and processing steps. In particular, the chemistry used for the site-specific probe immobilization together with that of the magnetic particles used for genome isolation should be PCR-compatible.

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