Selected article for: "agarose gel and dna template"
Author: Ayodeji, Mobolanle; Kulka, Michael; Jackson, Scott A; Patel, Isha; Mammel, Mark; Cebula, Thomas A; Goswami, Biswendu B
Title: A Microarray Based Approach for the Identification of Common Foodborne Viruses
  • Document date: 2009_3_19
    • ID: 7s5b3lpn_10
    Snippet: RNeasy Micro Kit (Qiagen, Valencia, CA); a mixture of oligo(dT 15 ) and random hexamers (pdN6) as primers; and AMV reverse transcriptase (Promega, Madison, WI) as previously described [4, 20] . In vitro transcribed and infected cell RNA templates represent in vitro and in vivo replication, respectively. PCR amplification with HAV or CV specific primers was carried out in 50 l reactions using 5 l of each RT reaction as template or 5ng of pHAV/7 pl.....
    Document: RNeasy Micro Kit (Qiagen, Valencia, CA); a mixture of oligo(dT 15 ) and random hexamers (pdN6) as primers; and AMV reverse transcriptase (Promega, Madison, WI) as previously described [4, 20] . In vitro transcribed and infected cell RNA templates represent in vitro and in vivo replication, respectively. PCR amplification with HAV or CV specific primers was carried out in 50 l reactions using 5 l of each RT reaction as template or 5ng of pHAV/7 plasmid DNA as previously described [20] . PCR products (5 l) were analyzed by agarose gel electrophoresis to confirm authenticity of product formation (data not shown).

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