Ebola Virus Epidemiology and Evolution in Nigeria

Folarin, Onikepe A.; Ehichioya, Deborah; Schaffner, Stephen F.; Winnicki, Sarah M.; Wohl, Shirlee; Eromon, Philomena; West, Kendra L.; Gladden-Young, Adrianne; Oyejide, Nicholas E.; Matranga, Christian B.; Deme, Awa Bineta; James, Ayorinde; Tomkins-Tinch, Christopher; Onyewurunwa, Kenneth; Ladner, Jason T.; Palacios, Gustavo; Nosamiefan, Iguosadolo; Andersen, Kristian G.; Omilabu, Sunday; Park, Daniel J.; Yozwiak, Nathan L.; Nasidi, Abdusallam; Garry, Robert F.; Tomori, Oyewale; Sabeti, Pardis C.; Happi, Christian T.

We prepared 16 samples from 13 of the 20 patients with confirmed EVD and discharge samples for 3 of them. This includes case 9, which could not be confidently matched to a sample (suspected match to E030). We prepared an additional 16 samples from suspected cases in which the sample could not be clearly associated with a particular case because of incomplete records. Dates and qRT-PCR results for each of these samples are reported in Supplementary Data 1. Because these data include retested and discharge samples, as well as incomplete information collated many months after the outbreak, we were not able to confirm that there were exactly 20 EVD cases in Nigeria. After inactivation and extraction at RUN, we divided RNA from each sample into 2 aliquots for independent library preparation and sequencing at RUN and the Broad Institute.