Title: Endoplasmic reticulum localization of Sec12p is achieved by two mechanisms: Rer1p-dependent retrieval that requires the transmembrane domain and Rer1p-independent retention that involves the cytoplasmic domain Document date: 1996_7_2
ID: 45x96b5d_13
Snippet: Escherichia coli strains used for recombinant DNA experiments were DH5ct (Sambrook et al., 1989) , SCS1 (Stratagene Cloning Systems), and XLI-Blue (Bullock et al., 1987) . DNA manipulations, including restriction enzyme digestions, ligations, plasmid isolation, and E. coli transformation, were carried out by standard methods (Sambrook et al., 1989) . In all constructions, DNA sequences were determined by the dideoxy method using a sequencer (mode.....
Document: Escherichia coli strains used for recombinant DNA experiments were DH5ct (Sambrook et al., 1989) , SCS1 (Stratagene Cloning Systems), and XLI-Blue (Bullock et al., 1987) . DNA manipulations, including restriction enzyme digestions, ligations, plasmid isolation, and E. coli transformation, were carried out by standard methods (Sambrook et al., 1989) . In all constructions, DNA sequences were determined by the dideoxy method using a sequencer (model 373A; Applied Biosystems, Tokyo, Japan). Yeast transformation was performed by the lithium thiocyanate method (Keszenman-Pereyra and Hieda, 1988) .
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