Author: MINAMI, Shohei; TERADA, Yutaka; SHIMODA, Hiroshi; TAKIZAWA, Masaki; ONUMA, Mamoru; OTA, Akihiko; OTA, Yuichi; AKABANE, Yoshihito; TAMUKAI, Kenichi; WATANABE, Keiichiro; NAGANUMA, Yumiko; KANAGAWA, Eiichi; NAKAMURA, Kaneichi; OHASHI, Masanari; TAKAMI, Yoshinori; MIWA, Yasutsugu; TANOUE, Tomoaki; OHWAKI, Masao; OHTA, Jouji; UNE, Yumi; MAEDA, Ken
Title: Establishment of serological test to detect antibody against ferret coronavirus Document date: 2016_3_3
ID: 3g75spkc_13
Snippet: Nucleotide sequence of the Yamaguchi-1 strain N gene (1,125 bp) was determined, and the deduced amino acid sequence of N protein (374 amino acids) was phylogenetically analyzed (Fig. 1) . Two recombinant N proteins, GST-N (1-179) and GST-N (180-374), based on the Yamaguchi-1 strain were expressed as GST fusion proteins in E. coli and used as ELISA antigens with 7 sera and 15 plasma samples from ferrets. Although most samples reacted to both recom.....
Document: Nucleotide sequence of the Yamaguchi-1 strain N gene (1,125 bp) was determined, and the deduced amino acid sequence of N protein (374 amino acids) was phylogenetically analyzed (Fig. 1) . Two recombinant N proteins, GST-N (1-179) and GST-N (180-374), based on the Yamaguchi-1 strain were expressed as GST fusion proteins in E. coli and used as ELISA antigens with 7 sera and 15 plasma samples from ferrets. Although most samples reacted to both recombinant proteins, the plasma of ferret No.10 and serum of ferret No.22 only reacted to GST-N (1-179) and did not recognize GST-N (180-374) (Fig. 2) . These results indicated that GST-N (1-179) was suitable for detection of antibodies to FRcoVs. Therefore, we de- cided to use GST-N (1-179) in the subsequent investigation.
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