Selected article for: "mini kit and RNA mini kit"
Author: MINAMI, Shohei; TERADA, Yutaka; SHIMODA, Hiroshi; TAKIZAWA, Masaki; ONUMA, Mamoru; OTA, Akihiko; OTA, Yuichi; AKABANE, Yoshihito; TAMUKAI, Kenichi; WATANABE, Keiichiro; NAGANUMA, Yumiko; KANAGAWA, Eiichi; NAKAMURA, Kaneichi; OHASHI, Masanari; TAKAMI, Yoshinori; MIWA, Yasutsugu; TANOUE, Tomoaki; OHWAKI, Masao; OHTA, Jouji; UNE, Yumi; MAEDA, Ken
Title: Establishment of serological test to detect antibody against ferret coronavirus
  • Document date: 2016_3_3
    • ID: 3g75spkc_5
    Snippet: Amplification of N genes: RNA of the Yamaguchi-1 strain was extracted from feces using a QIAamp Viral RNA Mini Kit (QIAGEN, Hilden, Germany) according to the manufacturer's instructions. N genes of the Yamaguchi-1 strain was amplified by RT-PCR using TaKaRa RNA LA PCR TM Kit (AMV) Ver. 1.1 (TaKaRa, Otsu, Japan). RT was performed using random 9-mer oligonucleotide primers, and PcR was performed using primer pairs, NF2 (5′-TTA CAT ATG GTA TAA GAA.....
    Document: Amplification of N genes: RNA of the Yamaguchi-1 strain was extracted from feces using a QIAamp Viral RNA Mini Kit (QIAGEN, Hilden, Germany) according to the manufacturer's instructions. N genes of the Yamaguchi-1 strain was amplified by RT-PCR using TaKaRa RNA LA PCR TM Kit (AMV) Ver. 1.1 (TaKaRa, Otsu, Japan). RT was performed using random 9-mer oligonucleotide primers, and PcR was performed using primer pairs, NF2 (5′-TTA CAT ATG GTA TAA GAA CTA AAC-3′) and NR2 (5′-CGA TGT AGG AAC CTT CAA AAT A-3′). PCR products were electrophoresed on a 0.8% gel and extracted using a QIAEX II Gel Extraction Kit (QIAGEN).

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