Author: Bancroft, Tara; DeBuysscher, Blair L.; Weidle, Connor; Schwartz, Allison; Wall, Abigail; Gray, Matthew D.; Feng, Junli; Steach, Holly R.; Fitzpatrick, Kristin S.; Gewe, Mesfin M.; Skog, Patrick D.; Doyle-Cooper, Colleen; Ota, Takayuki; Strong, Roland K.; Nemazee, David; Pancera, Marie; Stamatatos, Leonidas; McGuire, Andrew T.; Taylor, Justin J.
Title: Detection and activation of HIV broadly neutralizing antibody precursor B cells using anti-idiotypes Document date: 2019_10_7
ID: 63yvpuqx_55
Snippet: Non-V H 1-3-derived control antibodies were previous described Liao et al., 2013; McGuire et al., 2014a,b) . Paired heavy chain VDJ and light chain VJ sequences from BCR sequencing experiments or naive V H 1-3 + B cells identified previously were codon optimized and cloned into pTT3-derived expression vectors containing the human IgG1, IgK, or IgL constant regions using In-Fusion cloning (Clontech)......
Document: Non-V H 1-3-derived control antibodies were previous described Liao et al., 2013; McGuire et al., 2014a,b) . Paired heavy chain VDJ and light chain VJ sequences from BCR sequencing experiments or naive V H 1-3 + B cells identified previously were codon optimized and cloned into pTT3-derived expression vectors containing the human IgG1, IgK, or IgL constant regions using In-Fusion cloning (Clontech).
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