Author: Lee, Hyojin; Kim, Eun-Ju; Song, Jae-Young; Choi, Jeong Soo; Lee, Ji Youn; Cho, In-Soo; Shin, Yeun-Kyung
Title: Development and evaluation of a competitive enzyme-linked immunosorbent assay using a monoclonal antibody for diagnosis of severe fever with thrombocytopenia syndrome virus in bovine sera Document date: 2016_9_20
ID: 0pe8vgin_3
Snippet: Reverse transcriptase (RT)-polymerase chain reaction (PCR)-based methods have been conducted to detect the SFTSV antigen in blood samples [7, 16] . Moreover, immunofluorescence assays (IFA) are frequently used to detect SFTSV-specific antibodies in the sera of SFTS patients [23] . These diagnostic methods are associated with several disadvantages, such as the need to handle the live virus, which requires special facilities with high-level biosafe.....
Document: Reverse transcriptase (RT)-polymerase chain reaction (PCR)-based methods have been conducted to detect the SFTSV antigen in blood samples [7, 16] . Moreover, immunofluorescence assays (IFA) are frequently used to detect SFTSV-specific antibodies in the sera of SFTS patients [23] . These diagnostic methods are associated with several disadvantages, such as the need to handle the live virus, which requires special facilities with high-level biosafety equipment. Furthermore, these methods are laborious when applied to large numbers of samples [21] . In-house indirect enzyme-linked immunosorbent assays (ELISA) and double-antigen sandwich ELISA have been developed to detect immunoglobulins against the NP of SFTSV [5] .
Search related documents:
Co phrase search for related documents- high level and immunosorbent assay: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15
- high level and large number: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55
- high level and live virus: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15
- high level and reverse transcriptase: 1, 2, 3, 4, 5, 6, 7
- high level and special facility: 1
- IFA immunofluorescence assay and immunofluorescence assay: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74
- IFA immunofluorescence assay and immunosorbent assay: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
- IFA immunofluorescence assay and large number: 1, 2
- IFA immunofluorescence assay and live virus: 1, 2
- immunofluorescence assay and large number: 1, 2, 3
- immunofluorescence assay and live virus: 1, 2, 3, 4, 5, 6
- immunofluorescence assay and reverse transcriptase: 1, 2, 3, 4, 5, 6, 7
- immunosorbent assay and large number: 1, 2, 3, 4, 5, 6
- immunosorbent assay and live virus: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
- immunosorbent assay and reverse transcriptase: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35
- immunosorbent assay and SFTS patient: 1
- large number and live virus: 1, 2
- large number and reverse transcriptase: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16
- live virus and reverse transcriptase: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
Co phrase search for related documents, hyperlinks ordered by date