Author: Lee, Hyojin; Kim, Eun-Ju; Song, Jae-Young; Choi, Jeong Soo; Lee, Ji Youn; Cho, In-Soo; Shin, Yeun-Kyung
Title: Development and evaluation of a competitive enzyme-linked immunosorbent assay using a monoclonal antibody for diagnosis of severe fever with thrombocytopenia syndrome virus in bovine sera Document date: 2016_9_20
ID: 0pe8vgin_3
Snippet: Reverse transcriptase (RT)-polymerase chain reaction (PCR)-based methods have been conducted to detect the SFTSV antigen in blood samples [7, 16] . Moreover, immunofluorescence assays (IFA) are frequently used to detect SFTSV-specific antibodies in the sera of SFTS patients [23] . These diagnostic methods are associated with several disadvantages, such as the need to handle the live virus, which requires special facilities with high-level biosafe.....
Document: Reverse transcriptase (RT)-polymerase chain reaction (PCR)-based methods have been conducted to detect the SFTSV antigen in blood samples [7, 16] . Moreover, immunofluorescence assays (IFA) are frequently used to detect SFTSV-specific antibodies in the sera of SFTS patients [23] . These diagnostic methods are associated with several disadvantages, such as the need to handle the live virus, which requires special facilities with high-level biosafety equipment. Furthermore, these methods are laborious when applied to large numbers of samples [21] . In-house indirect enzyme-linked immunosorbent assays (ELISA) and double-antigen sandwich ELISA have been developed to detect immunoglobulins against the NP of SFTSV [5] .
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