Title: 2016 ACVIM Forum Research Abstract Program Document date: 2016_5_31
ID: 2y1y8jpx_320
Snippet: The presence of CP30 in 4 different feline Tf isolates was evaluated by indirect immunofluorescence and flow cytometry using a rabbit polyclonal antibody that targets bovine Tf CP30 (a-CP30). Feline Tf treated with rabbit IgG and/or secondary Ab in the absence of a-CP30 Ab and bovine Tf were used as negative and positive controls, respectively. The effect of inhibition of CP30 activity on Tf adhesion-dependent cytopathogenicity was determined usi.....
Document: The presence of CP30 in 4 different feline Tf isolates was evaluated by indirect immunofluorescence and flow cytometry using a rabbit polyclonal antibody that targets bovine Tf CP30 (a-CP30). Feline Tf treated with rabbit IgG and/or secondary Ab in the absence of a-CP30 Ab and bovine Tf were used as negative and positive controls, respectively. The effect of inhibition of CP30 activity on Tf adhesion-dependent cytopathogenicity was determined using CFSE-labeled feline Tf that were allowed to adhere to monolayers of porcine intestinal epithelial cells (IPEC) in coculture. The effect of CP30 inhibition on feline Tf adhesion and cytotoxicity was evaluated by immunofluorescence quantification assays and crystal violet spectrophotometric analysis. A minimum of 6 replicates were performed for each co-culture experiment. Data were analyzed using Systat software (P < 0.05).
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