Author: Ivanova, Elena; Berger, Audrey; Scherrer, Anne; Alkalaeva, Elena; Strub, Katharina
Title: Alu RNA regulates the cellular pool of active ribosomes by targeted delivery of SRP9/14 to 40S subunits Document date: 2015_3_11
ID: 64cnoqpi_19
Snippet: HEK293T cells were transfected with pDL7enh, pDLscAlu or pDL4.5S and 24 h later infected with VSV-PeGFP (50) at a multiplicity of infection of 10 PFU/cell in Dulbecco's modified Eagle's medium (DMEM) 2% fetal bovine serum (FBS). Six hours post-infection, the media was changed and cells were pulse labeled with [ 35 S]-methionine/cysteine for 15 min, proteins were precipitated in 10% TCA, resolved by SDS-PAGE and quantified by phosphorimager analys.....
Document: HEK293T cells were transfected with pDL7enh, pDLscAlu or pDL4.5S and 24 h later infected with VSV-PeGFP (50) at a multiplicity of infection of 10 PFU/cell in Dulbecco's modified Eagle's medium (DMEM) 2% fetal bovine serum (FBS). Six hours post-infection, the media was changed and cells were pulse labeled with [ 35 S]-methionine/cysteine for 15 min, proteins were precipitated in 10% TCA, resolved by SDS-PAGE and quantified by phosphorimager analysis.
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