Selected article for: "AluY presence and mRNA recruitment"
Author: Ivanova, Elena; Berger, Audrey; Scherrer, Anne; Alkalaeva, Elena; Strub, Katharina
Title: Alu RNA regulates the cellular pool of active ribosomes by targeted delivery of SRP9/14 to 40S subunits
  • Document date: 2015_3_11
    • ID: 64cnoqpi_36
    Snippet: Next, we wanted to address the question whether Alu RNPs interfere with the formation of the 48S complex, which is to say with the recruitment of the mRNA to the 43S complex, or whether they already inhibit 43S complex formation. To this end, we reconstituted 43S and 48S complexes from purified components in the presence of the AluY A or scAluY A RNPs and as a negative control we used the SA110 RNP. Formation of 43S and 48S complexes was performe.....
    Document: Next, we wanted to address the question whether Alu RNPs interfere with the formation of the 48S complex, which is to say with the recruitment of the mRNA to the 43S complex, or whether they already inhibit 43S complex formation. To this end, we reconstituted 43S and 48S complexes from purified components in the presence of the AluY A or scAluY A RNPs and as a negative control we used the SA110 RNP. Formation of 43S and 48S complexes was performed in the presence of [ 35 S]-Met-tRNA i Met or [ 32 P]-labeled MVHLstop mRNA, respectively, and the assembly reactions were fractionated by sucrose gradient centrifugation to monitor the distribution of the radioactive components.

    Search related documents:
    Co phrase search for related documents
    • Alu rnp and mRNA recruitment: 1
    • Alu rnp and SA110 RNP: 1
    • AluY presence and assembly reaction: 1
    • AluY presence and complex formation: 1, 2
    • AluY presence and mRNA recruitment: 1
    • AluY presence and SA110 RNP: 1
    • assembly reaction and SA110 RNP: 1
    • complex formation and gradient centrifugation: 1, 2
    • complex formation and mRNA recruitment: 1, 2, 3, 4
    • complex formation and negative control: 1
    • complex formation and question address: 1
    • complex formation and SA110 RNP: 1, 2, 3
    • complex formation and sucrose gradient centrifugation: 1, 2
    • gradient centrifugation and negative control: 1, 2
    • gradient centrifugation and sucrose gradient centrifugation: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • negative control and sucrose gradient centrifugation: 1