Author: Leung, Ho-Chuen; Chan, Chris Chung-Sing; Poon, Vincent Kwok-Man; Zhao, Han-Jun; Cheung, Chung-Yan; Ng, Fai; Huang, Jian-Dong; Zheng, Bo-Jian
Title: An H5N1-based matrix protein 2 ectodomain tetrameric peptide vaccine provides cross-protection against lethal infection with H7N9 influenza virus Document date: 2015_4_8
ID: 14qckds8_10
Snippet: Virus titers were detected by TCID 50 assay as described previously. 20 Briefly, the infected allantoic fluid and mouse lung homogenates were serially diluted 10-fold in minimum essential medium (Invitrogen, Waltham, MA, USA) containing a cocktail of antibiotics. The diluted samples were added to PBS-prewashed Madin-Darby canine kidney cell monolayer in a 96-well plate. The cell cultures were incubated at 37 6 C for 1 h, and the supernatants were.....
Document: Virus titers were detected by TCID 50 assay as described previously. 20 Briefly, the infected allantoic fluid and mouse lung homogenates were serially diluted 10-fold in minimum essential medium (Invitrogen, Waltham, MA, USA) containing a cocktail of antibiotics. The diluted samples were added to PBS-prewashed Madin-Darby canine kidney cell monolayer in a 96-well plate. The cell cultures were incubated at 37 6 C for 1 h, and the supernatants were replaced with fresh minimum essential medium containing a cocktail of antibiotics. The cytopathic effect that appeared in the infected Madin-Darby canine kidney cells was observed daily and recorded on the third-day post-infection.
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