Selected article for: "cdna dna PCR amplification and PCR amplification"
Author: Draz, Mohamed Shehata; Shafiee, Hadi
Title: Applications of gold nanoparticles in virus detection
  • Document date: 2018_2_15
    • ID: 1xjmlwqr_71
    Snippet: One AuNP-based colorimetric assay has been reported for the molecular detection of HEV [152] . This assay targets a conservative fragment of HEV in ORF1 that is pre-amplified by one-step RT-PCR/PCR amplification, and the generated cDNA is labeled by AuNP-DNA conjugates, forming three-component sandwich hybrids in a manner very similar to that previously depicted in Fig. 6 . This hybridization is followed by a silver-staining step to allow the sca.....
    Document: One AuNP-based colorimetric assay has been reported for the molecular detection of HEV [152] . This assay targets a conservative fragment of HEV in ORF1 that is pre-amplified by one-step RT-PCR/PCR amplification, and the generated cDNA is labeled by AuNP-DNA conjugates, forming three-component sandwich hybrids in a manner very similar to that previously depicted in Fig. 6 . This hybridization is followed by a silver-staining step to allow the scanometric detection of target DNA either by the naked eye or using simple scanners, with a detection limit of 100 fM of HEV amplicon.

    Search related documents:
    Co phrase search for related documents
    • detection limit and naked eye: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • detection limit and RT PCR PCR amplification: 1, 2, 3
    • detection limit and sandwich hybrid: 1
    • detection limit and scanometric detection: 1, 2, 3
    • HEV molecular detection and molecular detection: 1
    • molecular detection and naked eye: 1, 2, 3, 4, 5
    • molecular detection and scanometric detection: 1, 2
    • naked eye and scanometric detection: 1