Author: Draz, Mohamed Shehata; Shafiee, Hadi
Title: Applications of gold nanoparticles in virus detection Document date: 2018_2_15
ID: 1xjmlwqr_71
Snippet: One AuNP-based colorimetric assay has been reported for the molecular detection of HEV [152] . This assay targets a conservative fragment of HEV in ORF1 that is pre-amplified by one-step RT-PCR/PCR amplification, and the generated cDNA is labeled by AuNP-DNA conjugates, forming three-component sandwich hybrids in a manner very similar to that previously depicted in Fig. 6 . This hybridization is followed by a silver-staining step to allow the sca.....
Document: One AuNP-based colorimetric assay has been reported for the molecular detection of HEV [152] . This assay targets a conservative fragment of HEV in ORF1 that is pre-amplified by one-step RT-PCR/PCR amplification, and the generated cDNA is labeled by AuNP-DNA conjugates, forming three-component sandwich hybrids in a manner very similar to that previously depicted in Fig. 6 . This hybridization is followed by a silver-staining step to allow the scanometric detection of target DNA either by the naked eye or using simple scanners, with a detection limit of 100 fM of HEV amplicon.
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