Selected article for: "detection limit and ELISA detection"

Author: Draz, Mohamed Shehata; Shafiee, Hadi
Title: Applications of gold nanoparticles in virus detection
  • Document date: 2018_2_15
  • ID: 1xjmlwqr_96
    Snippet: Finally, the immunological detection of H1N1 hemagglutinin (HA) antigen has only been reported through a novel LSPR-based immunoassay. In this assay, the presence of target antigen induces a sandwich immunoreaction between the fluorophorelabeled detection antibodies and the AuNP-capturing antibody conjugates, directly resulting in enhanced fluorescence caused by the coupling of fluorophore emission with the localized surface plasmons of AuNPs in .....
    Document: Finally, the immunological detection of H1N1 hemagglutinin (HA) antigen has only been reported through a novel LSPR-based immunoassay. In this assay, the presence of target antigen induces a sandwich immunoreaction between the fluorophorelabeled detection antibodies and the AuNP-capturing antibody conjugates, directly resulting in enhanced fluorescence caused by the coupling of fluorophore emission with the localized surface plasmons of AuNPs in a manner similar to that shown in Fig. 9C . The sensitivity of this method has been described to be 10 3 -fold, improved over that of the conventional ELISA technique, with a reported limit of detection of 13.9 pg/mL [63] .

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