Author: Klem, T. B.; Tollersrud, T.; Østerås, O.; Stokstad, M.
Title: Association between the level of antibodies in bulk tank milk and bovine respiratory syncytial virus exposure in the herd Document date: 2014_7_12
ID: 0niak4oy_4
Snippet: Several methods are available for detection of antibodies against BRSV, including virus neutralisation tests and different ELISAs, of which the indirect ELISA is most commonly used. In a neutralisation test, the level of antibodies in a sample is measured quantitatively. The indirect ELISA is not developed as a quantitative assay; its function is primarily to differentiate between negative and positive samples, and to be semiquantitative at antib.....
Document: Several methods are available for detection of antibodies against BRSV, including virus neutralisation tests and different ELISAs, of which the indirect ELISA is most commonly used. In a neutralisation test, the level of antibodies in a sample is measured quantitatively. The indirect ELISA is not developed as a quantitative assay; its function is primarily to differentiate between negative and positive samples, and to be semiquantitative at antibody levels where seroconversion in individual animals is usually seen. Outside this range, there may be a weaker correlation between the ELISA optical density (OD) values and the actual level of antibodies in the sample.
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