Author: Banu, Nasirah; Chia, Adeline; Ho, Zi Zong; Garcia, Alfonso Tan; Paravasivam, Komathi; Grotenbreg, Gijsbert M.; Bertoletti, Antonio; Gehring, Adam J.
Title: Building and Optimizing a Virus-specific T Cell Receptor Library for Targeted Immunotherapy in Viral Infections Document date: 2014_2_25
ID: 44w6omdp_13
Snippet: We then tested whether the inflammatory environment led to increased Th1 polarization or caused non-specific bystander activation to account for the increase in IFN-c1 T cells. Unlike figure 4 , where HLA-pentamer and IFN-c staining were performed in separate assays, we measured IFN-c production in HLA pentamer1 or TCR Vbeta1 cells to measure cytokine production in TCR transduced cells......
Document: We then tested whether the inflammatory environment led to increased Th1 polarization or caused non-specific bystander activation to account for the increase in IFN-c1 T cells. Unlike figure 4 , where HLA-pentamer and IFN-c staining were performed in separate assays, we measured IFN-c production in HLA pentamer1 or TCR Vbeta1 cells to measure cytokine production in TCR transduced cells.
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